Sperm DNA Fragmentation index in a male with type I globozoospermia (total globozoospermia)

Authors

DOI:

https://doi.org/10.37800/RM.4.2021.30-35

Keywords:

type I and type II globozoospermia, acrosome formation, DNA Fragmentation index, Sperm Chromatin Structure Assay (SCSA), direct swim-up, density gradient

Abstract

Relevance: Total globozoospermia (Type 1) is a severe teratozoospermia leading to complete male infertility. It is an extremely rare spermatozoa pathology caused by disruption of acrosome formation.

The study aimed to determine and compare DNA fragmentation test results in a patient with a total globozoospermia after several sperm processing methods.

Methods: This article describes a case of a male with total globozoospermia. This study examined the DNA Fragmentation Index by Sperm Chromatin Structure Assay (SCSA) of a patient with type I globozoospermia. We compared the test results before and after processing the ejaculate with several sperm processing methods described in the WHO laboratory manual for the examination and processing of human semen (2010).

Results: SCSA showed no abnormality in DNA Fragmentation in the patient with total globozoospermia. DNA Fragmentation index did not improve significantly after processing the sperm with a direct swim-up method and discontinuous density gradient.

Conclusion: According to SCSA results, the acrosome absence did not affect DNA stability in a patient with total globozoospermia.

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Additional Files

Published

2021-12-30

How to Cite

[1]
Zhumadil Х., Magauiya А. , Bekbosynova Г., Abishev А., Yermekov М., Umorbekova Г. and Begaidarova А. 2021. Sperm DNA Fragmentation index in a male with type I globozoospermia (total globozoospermia). Reproductive Medicine. 4 (49) (Dec. 2021), 29–34. DOI:https://doi.org/10.37800/RM.4.2021.30-35.

Issue

No. 4 (49) (2021): Reproductive medicine

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Статьи

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