Endometrium culture of the human: research of invasive
Keywords:
culture of human endometrial cells, endometriosis, cell culture assay, invasionAbstract
External genital endometriosis is a female gynecological disease characterized by tissue growth similar to endometrium
outside the uterus. This is due to the proteolysis properties of the stroma of the uterine lining. Migration and invasion are key
properties of living cells and play a crucial role in the normal development of the body, the immune response and pathological
processes such as cancer metastasis and inflammatory processes. In our study, an analysis of cell culture invasion was carried
out, based on the measurement of cell motility and the activity of cell movement along a chemio-attractant gradient. It was
shown that in the control the number of cells that passed through the transerobny filter and adhered to the surface varied from 4.6 to 16.3.
In the cell culture obtained with endometriosis, these figures were almost the same and were 6.5–12.8. The addition of
peritoneal fluid to the lower chamber did not affect the migration abilities of the cells of the control group, however, it enhanced invasion in the group with endometriosis.
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