Improved sperm selection strategies as a treatment for infertility
DOI:
https://doi.org/10.37800/RM.4.2023.18-22Keywords:
Microfluidics, Sperm, Infertility, Intracytoplasmic Sperm Injection (ICSI), Intracytoplasmic Morphological Sperm Injection (IMSI), Physiological Intracytoplasmic Sperm Injection (PICSI), Magnetic-Activated Cell Sorting (MACS)Abstract
Relevance: Sperm selection is an important step in any ART program as it ensures that the highest quality gametes are used for fertilization, increasing the chances of a positive outcome.
The study aimed to compare traditional and improved sperm selection methods and evaluate their impact on the embryological parameters of ART programs.
Materials and Methods: The study included 609 ICSI cycles that used different advanced approaches to sperm selection, including traditional ICSI (control group), morphological ICSI (IMSI), physiological ICSI (PICSI), microfluidic and magnetic- activated sperm selection (MACS). Statistical analysis was performed using GraphPad Prism 9.5.1 software. A significance level of P≤0.05 was considered significant.
Results: According to the data obtained, improved approaches to sperm selection lead to improved embryological parameters of ART programs. Morphological, microfluidic, and magnetic selection significantly increased the number of fertilized oocytes compared to the control group (p=0.0122; p=0.0074; p=0.0011, respectively). Microfluidic and magnetic selection demonstrated a significant increase in total blastocyst formation compared to the control group (p=0.0051; p=0.0022, respectively). The number of blastocysts of good and excellent quality (AA, BA, AB, BB, expansion degree from 2 and higher) increased significantly in all experimental groups (p<0.0001 for IMSI, PICSI, MACS and microfluidic selection). There were no significant differences in fertilization and blastocyst formation numbers between the improved sperm selection methods.
Conclusion: The effectiveness of advanced approaches in sperm selection did not differ significantly. Identifying the specific clinical conditions and indications for which a particular method would be most useful is necessary.
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