Late blastulation in human embryos and its impact on pregnancy outcome for patients undergoing treatment in an ART clinic
DOI:
https://doi.org/10.37800/RM.4.2023.13-17Keywords:
Blastocyst, late blastulation, 7 day blastocysts, vitrification, PGT-A, pregnancy rateAbstract
Relevance: It is already known that embryos can give birth on the 7th day of development. However, published data about the fertility outcomes of transferring embryos with a delayed blastulation on Day 7 are scarce and controversial. Moreover, few data are available on the neonatal outcomes of Day 7 blastocysts. As a result, the clinical value of Day 7 blastocysts is uncertain for any category of patients.
The study aimed to analyze our own data to determine further tactics regarding late blastulation (blastocysts on the 7th day of development) and the possibility of using such embryos for different age groups of patients.
Materials and Methods: The analysis of reproductive potential included blastocysts, cryopreserved by vitrification day 7 of development and transferred in cryocycles from 2013 to 2022 at AVA-PETER Ltd. (St. Petersburg, Russia). The study included patients of three age groups: Group I – below 34 years, Group II – 35-39 years, and Group III – 40+ years. The control group included 3985 Day 5 embryos. All data were analyzed using the chi-square test.
Results: The average age of patients who received day 7 blastocysts was higher than those in the control group. Blastocysts vitrified on day 7 had a significantly lower implantation potential than blastocysts vitrified on day 5. Morphological qualities of day 7 embryos significantly influenced the transfer outcome. Pregnancy rates when transferring day 7 embryos did not differ statistically with or without PGT-A.
Conclusion: Blastocysts of the 7th day of development of quality AA, AB, and BA can be recommended for transfer. Genetic testing does not increase the pregnancy rate for day 7 blastocysts.
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