Age-related determinants of copper and manganese levels in women of the Aktobe region
DOI:
https://doi.org/10.37800/RM.2.2026.789Keywords:
trace elements, fertility, women, age group, pregnancyAbstract
Relevance: The significance of necessary and harmful trace elements in regulating female reproductive function has been intensively investigated in recent years. There is increasing evidence that the pathophysiology of gynecological and metabolic illnesses is associated with disturbances in trace element homeostasis.
The study aimed to assess the elemental status of women of reproductive age in the Aktobe region (Kazakhstan) and determine its relationship with age characteristics.
Materials and methods: A cross-sectional study was conducted involving women aged 18-35 years (n = 281). Concentrations of trace elements (cadmium, cobalt, lead, copper, manganese, selenium, zinc, chromium, magnesium) were determined using atomic absorption spectrometry. Statistical analysis was performed using SPSS version 26.0. Normality of distribution was assessed using the Kolmogorov-Smirnov test; data are presented as median and interquartile range (Q1-Q3). The Mann-Whitney U test was used for intergroup comparisons. Participants were divided into two age groups: 18-25 years and 25-35 years. Correlation analysis was performed using Spearman’s rank correlation coefficient.
Results: No statistically significant differences were found between age groups for most trace elements (cadmium, cobalt, lead, selenium, zinc, chromium, magnesium) (p > 0.05). However, significant differences were observed for copper and manganese: copper levels were 1.6 times higher in the second group (p < 0.001), and manganese levels were 2 times higher (p = 0.004). Correlation analysis revealed a moderate positive association between age and copper levels (r = 0.339; p < 0.001) and a weak positive association with manganese levels (r = 0.183; p = 0.002).
Conclusion: Age-related changes in trace element status among women of reproductive age (25-35 years) are limited. Copper and manganese may be considered potential indicators reflecting age-related metabolic and biological characteristics. These findings highlight the need for further research into the role of trace elements in the regulation of women’s reproductive health.
References
1. Kocić J, Zečević N, Jagodić J, Ardalić D, Miković Ž, Kotur-Stevuljević J, Manojlović D, Stojsavljević A. Exploring serum trace element shifts: implications for cervical intraepithelial neoplasia. J Trace Elem Med Biol. 2024;86:127531.
https://doi.org/10.1016/j.jtemb.2024.127531
2. Kocić J, Zečević N, Jagodić J, Mihajlović D, Dzuverović M, Pavlović N, Kotur-Stevuljević J, Manojlović D, Stojsavljević A. Impact of essential and toxic trace elements on cervical premalignant lesions. Biomedicines. 2025;13(12):3015.
https://doi.org/10.3390/biomedicines13123015
3. Michalczyk K, Kapczuk P, Kupnicka P, Witczak G, Michalczyk B, Bosiacki M, Chlubek D, Cymbaluk-Płoska A. Assessment of serum Zn, Cu, Mn, and Fe concentration in women with endometrial cancer and different endometrial pathologies. Nutrients. 2023;15(16):3605.
https://doi.org/10.3390/nu15163605
4. Wang X, Grubb DM, O’Mara TA. Dietary Factors and Endometrial Cancer Risk: A Mendelian Randomization Study. Nutrients. 2023;15(3):603.
https://doi.org/10.3390/nu15030603
5. Knažická Z, Bihari M, Janсo I, Harangozo Ľ, Árvay J, Kováčik A, Massányi P, Gálik B, Saraiva JMA, Habanova M. Blood Concentration of Macro- and Microelements in Women Who Are Overweight/Obesity and Their Associations with Serum Biochemistry. Life (Basel). 2024;14(4):465.
https://doi.org/10.3390/life14040465
6. Li M, Tan Y, Lin C, Huang Q, Lei D, Hu Y. Serum Macroelement and Microelement Concentrations in Patients with Polycystic Ovary Syndrome: a Cross-Sectional Study. Biol Trace Elem Res. 2017;176(1):73-80. Epub 2016.
https://doi.org/10.1007/s12011-016-0782-4
7. Dong R, Ren S, Wang X, Yuan J, Yin T, Zhang D, Li Q, Yang J, Sun Z, Liu Y, Yang L, Tao F, Zhang Z, Ji D, Wei J, Liang C. Cysteine mediates the association of essential trace elements with polycystic ovary syndrome: Does ambient particulate matters exposure modify the mediation effect of cysteine. Ecotoxicol Environ Saf. 2025 September 15;303:118868.
https://doi.org/10.1016/j.ecoenv.2025.118868.
8. Mohsin R, Fujimoto VY, Galusha AL, Parsons PJ, Krall JR, Butts-Jackson CD, Mok-Lin E, Bloom MS. Associations between follicular fluid trace elements and ovarian response during in vitro fertilization. Environ Res. 2024;252(Pt 1):118801.
https://doi.org/10.1016/j.envres.2024.118801
9. Watson CV, Lewin M, Ragin-Wilson A, Jones R, Jarrett JM, Wallon K, Ward C, Hilliard N, Irvin-Barnwell E. Characterization of trace elements exposure in pregnant women in the United States, NHANES 1999-2016. Environ Res. 2020;183:109208.
https://doi.org/10.1016/j.envres.2020.109208
10. Geller RJ, Wesselink AK, Upson K, Claus Henn B, Schildroth S, Wright R, Coleman CM, Willis MD, Bethea TN, Williams PL, Harmon QE, Baird DD, Wegienka G, Wise LA. Correlates of whole blood metal concentrations among reproductive-aged Black women. J Expo Sci Environ Epidemiol. 2023;33(6):883-894. Epub 2022.
https://doi.org/10.1038/s41370-022-00477-y
11. Tian T, Wang Z, Liu F, Fu Y, Kong F, Wang Y, Li Q, Long X, Qiao J. Exposure to heavy metallic and trace essential elements and risk of diminished ovarian reserve in reproductive age women: a case-control study. J Hazard Mater. 2024;470:134206.
https://doi.org/10.1016/j.jhazmat.2024.134206
12. Li D, Jiang T, Wang X, Yin T, Shen L, Zhang Z, Zou W, Liu Y, Zong K, Liang D, Cao Y, Xu X, Liang C, Ji D. Serum essential trace element status in women and the risk of endometrial diseases: a case-control study. Biol Trace Elem Res. 2023;201(5):2151-2161. Epub 2022.
https://doi.org/10.1007/s12011-022-03328-x
13. Butts CD, Bloom MS, McGough A, Lenhart N, Wong R, Mok-Lin E, Parsons PJ, Galusha AL, Yucel RM, Feingold BJ, Browne RW, Fujimoto VY. Variability of essential and non-essential trace elements in follicular fluid among women undergoing in vitro fertilization (IVF). Ecotoxicol Environ Saf. 2021;209:111733.
https://doi.org/10.1016/j.ecoenv.2020.111733
14. Sharma P, Kapoor HS, Kaur B, Kamra P, Khetarpal P. Investigation of the association of serum trace element concentrations and serum biochemical parameters with the risk of polycystic ovary syndrome: a case-control study. Biol Trace Elem Res. 2024;202(1):73-86. Epub 2023.
https://doi.org/10.1007/s12011-023-03664-6
15. López-Botella A, Sánchez R, Todolí-Torró JL, Gómez-Torres MJ, Velasco I, Acién M. A new advanced and validated method for the determination of potentially toxic metals and micro- and ultra-trace elements in peritoneal fluid for the assessment of health risks in women with gynecological pathologies. Toxics. 2023;11(5):399.
https://doi.org/10.3390/toxics11050399
16. Amanzholkyzy A, Sagidanova Y, Stankevicius E, Donayeva A, Sarsengali U. Heavy metal toxicity versus trace element protection in women’s reproductive health: a systematic review. Georgian Med News. 2025;(369):210-216.
https://pubmed.ncbi.nlm.nih.gov/41687657/
17. Choi R, Sun J, Yoo H, Kim S, Cho YY, Kim HJ, Kim SW, Chung JH, Oh SY, Lee SY. A prospective study of serum trace elements in healthy Korean pregnant women. Nutrients. 2016;8(11):749.
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